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Primary antibodies used in this study.
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Primary antibodies used in this study.
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Primary antibodies used in this study.
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Primary antibodies used in this study.
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Image Search Results


Primary antibodies used in this study.

Journal: Biology of Reproduction

Article Title: Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice †

doi: 10.1093/biolre/iox150

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: 1:500 n/a POLR2A Mo Abcam Ab32485 AB_732124 1:100 n/a RPS3 Rab Thermo PA5-27974 AB_2545450 1:100 n/a RPS6 Mo Thermo MA5-15123 AB_10999800 1:100 n/a RPS13 Rab Abcam Ab104862 AB_10712319 1:100 n/a RPL10A Mo Santa Cruz sc-100827 AB_2285311 1:100 n/a RPL10L Mo Abnova 115–214 AB_10864171 1:50 n/a RPL28 Go Santa Cruz sc-14151 AB_2181749 1:100 n/a EIF4EBP1 Go Santa Cruz sc-6025 AB_633426 1:100 n/a AGO4 Go Santa Cruz sc-32664 AB_2277670 1:100 n/a MKNK1 Rab Cell Signaling 2195S AB_2235175 1:100 n/a MKNK2 Rab Abcam Ab84345 AB_1860814 1:100 n/a PABPC1 Rab Abcam Ab21060 AB_777008 1:100 1:1000 BOLL Rab Thermo PA5-30808 AB_2548282 1:150 n/a DAZL Rab Cell signaling 8042S AB_10860078 1:150 1:2000 ELAVL1 Rab Millipore 07-468 AB_310641 1:200 1:2000 MSI1 Go R&D AF2628 AB_2147926 1:50 n/a TSN Rab Abcam Ab71775 AB_1524509 1:50 n/a HSPA2 Rab ProteinTech 12797-1-AP AB_2119687 1:500 n/a pCDK1 Rab Thermo MA5-15062 AB_10984869 1:50 n/a pCDK2 Rab Cell signaling 2561S AB_2078685 1:100 n/a pCHEK1 Rab Cell signaling 2341S AB_330023 1:100 n/a pCHEK2 Rab Abcam Ab59408 AB_942224 1:100 n/a p4EBP1(S64) Rab Cell Signaling 9451S AB_330947 1:100 n/a p4EBP1(T69) Rab Cell Signaling 9455S AB_330949 1:50 n/a p4EBP1(S111) Rab Abgent AP3473a AB_1278020 1:50 n/a Puromycin Mo Millipore MABE343 AB_2566826 1:10 000 n/a TUBA1A Mo Sigma T9026 AB_477593 1:500 1:5000 TUBG1 Go Santa Cruz sc-7396 AB_2211262 1:200 n/a FLAG Mo Sigma F1804 AB_262044 1:200 n/a Open in a separate window Primary antibodies used in this study.

Techniques:

Some translational factors are relatively enriched in the XY body of pachytene spermatocytes. (A-D) EIF4F complex members EIF4A1, EIF4E, EIF4E2, and phosphorylated EIF4E were found in the XY body. (E-H) Small ribosomal subunit protein RPS6 and large ribosomal subunit protein RPL10L, and RNA-binding proteins MSI1 and TSN were relatively enriched in the XY body. (I-L) Translational activators MKNK1 and MKNK2, and repressors EIF4EBP1 and EIF2C4 (AGO4), were found in the XY body. Bars = 5 μm.

Journal: Biology of Reproduction

Article Title: Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice †

doi: 10.1093/biolre/iox150

Figure Lengend Snippet: Some translational factors are relatively enriched in the XY body of pachytene spermatocytes. (A-D) EIF4F complex members EIF4A1, EIF4E, EIF4E2, and phosphorylated EIF4E were found in the XY body. (E-H) Small ribosomal subunit protein RPS6 and large ribosomal subunit protein RPL10L, and RNA-binding proteins MSI1 and TSN were relatively enriched in the XY body. (I-L) Translational activators MKNK1 and MKNK2, and repressors EIF4EBP1 and EIF2C4 (AGO4), were found in the XY body. Bars = 5 μm.

Article Snippet: 1:500 n/a POLR2A Mo Abcam Ab32485 AB_732124 1:100 n/a RPS3 Rab Thermo PA5-27974 AB_2545450 1:100 n/a RPS6 Mo Thermo MA5-15123 AB_10999800 1:100 n/a RPS13 Rab Abcam Ab104862 AB_10712319 1:100 n/a RPL10A Mo Santa Cruz sc-100827 AB_2285311 1:100 n/a RPL10L Mo Abnova 115–214 AB_10864171 1:50 n/a RPL28 Go Santa Cruz sc-14151 AB_2181749 1:100 n/a EIF4EBP1 Go Santa Cruz sc-6025 AB_633426 1:100 n/a AGO4 Go Santa Cruz sc-32664 AB_2277670 1:100 n/a MKNK1 Rab Cell Signaling 2195S AB_2235175 1:100 n/a MKNK2 Rab Abcam Ab84345 AB_1860814 1:100 n/a PABPC1 Rab Abcam Ab21060 AB_777008 1:100 1:1000 BOLL Rab Thermo PA5-30808 AB_2548282 1:150 n/a DAZL Rab Cell signaling 8042S AB_10860078 1:150 1:2000 ELAVL1 Rab Millipore 07-468 AB_310641 1:200 1:2000 MSI1 Go R&D AF2628 AB_2147926 1:50 n/a TSN Rab Abcam Ab71775 AB_1524509 1:50 n/a HSPA2 Rab ProteinTech 12797-1-AP AB_2119687 1:500 n/a pCDK1 Rab Thermo MA5-15062 AB_10984869 1:50 n/a pCDK2 Rab Cell signaling 2561S AB_2078685 1:100 n/a pCHEK1 Rab Cell signaling 2341S AB_330023 1:100 n/a pCHEK2 Rab Abcam Ab59408 AB_942224 1:100 n/a p4EBP1(S64) Rab Cell Signaling 9451S AB_330947 1:100 n/a p4EBP1(T69) Rab Cell Signaling 9455S AB_330949 1:50 n/a p4EBP1(S111) Rab Abgent AP3473a AB_1278020 1:50 n/a Puromycin Mo Millipore MABE343 AB_2566826 1:10 000 n/a TUBA1A Mo Sigma T9026 AB_477593 1:500 1:5000 TUBG1 Go Santa Cruz sc-7396 AB_2211262 1:200 n/a FLAG Mo Sigma F1804 AB_262044 1:200 n/a Open in a separate window Primary antibodies used in this study.

Techniques: RNA Binding Assay

Primary antibodies used in this study.

Journal: Biology of Reproduction

Article Title: Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice †

doi: 10.1093/biolre/iox150

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: 1:500 n/a POLR2A Mo Abcam Ab32485 AB_732124 1:100 n/a RPS3 Rab Thermo PA5-27974 AB_2545450 1:100 n/a RPS6 Mo Thermo MA5-15123 AB_10999800 1:100 n/a RPS13 Rab Abcam {"type":"entrez-nucleotide","attrs":{"text":"Ab104862","term_id":"82617533","term_text":"AB104862"}} Ab104862 AB_10712319 1:100 n/a RPL10A Mo Santa Cruz sc-100827 AB_2285311 1:100 n/a RPL10L Mo Abnova 115–214 AB_10864171 1:50 n/a RPL28 Go Santa Cruz sc-14151 AB_2181749 1:100 n/a EIF4EBP1 Go Santa Cruz sc-6025 AB_633426 1:100 n/a AGO4 Go Santa Cruz sc-32664 AB_2277670 1:100 n/a MKNK1 Rab Cell Signaling 2195S AB_2235175 1:100 n/a MKNK2 Rab Abcam Ab84345 AB_1860814 1:100 n/a PABPC1 Rab Abcam Ab21060 AB_777008 1:100 1:1000 BOLL Rab Thermo PA5-30808 AB_2548282 1:150 n/a DAZL Rab Cell signaling 8042S AB_10860078 1:150 1:2000 ELAVL1 Rab Millipore 07-468 AB_310641 1:200 1:2000 MSI1 Go R&D AF2628 AB_2147926 1:50 n/a TSN Rab Abcam Ab71775 AB_1524509 1:50 n/a HSPA2 Rab ProteinTech 12797-1-AP AB_2119687 1:500 n/a pCDK1 Rab Thermo MA5-15062 AB_10984869 1:50 n/a pCDK2 Rab Cell signaling 2561S AB_2078685 1:100 n/a pCHEK1 Rab Cell signaling 2341S AB_330023 1:100 n/a pCHEK2 Rab Abcam Ab59408 AB_942224 1:100 n/a p4EBP1(S64) Rab Cell Signaling 9451S AB_330947 1:100 n/a p4EBP1(T69) Rab Cell Signaling 9455S AB_330949 1:50 n/a p4EBP1(S111) Rab Abgent AP3473a AB_1278020 1:50 n/a Puromycin Mo Millipore MABE343 AB_2566826 1:10 000 n/a TUBA1A Mo Sigma T9026 AB_477593 1:500 1:5000 TUBG1 Go Santa Cruz sc-7396 AB_2211262 1:200 n/a FLAG Mo Sigma F1804 AB_262044 1:200 n/a Open in a separate window Primary antibodies used in this study.

Techniques: